Mattingly also said that catcher A.J. Ellis was sent home with the flu.
Crawford said the leg tightened up as he scored from first base on Yasiel Puig's double in the third inning of the Cactus League opener against Arizona on Wednesday.
Crawford spent a month on the disabled list last year with a strained left hamstring.
Ken Gurnick is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.