Jerry Hairston got the nod at second base, while Adam Kennedy was penciled in at third and Matt Treanor started behind the plate.
Tony Gwynn also got his second straight start in left field, giving Juan Rivera the night off.
"With a guy that we don't really know, I feel like we are better off with a left-handed lineup," Mattingly said. "Instead of doing everyone tomorrow, I figured I would break it up and give some today off and others tomorrow off.
"Jerry is most comfortable at second and Adam can play second and third."
Mattingly also fully recognizes which players need more rest than others during the grind of a long season.
"It was either going to be today or tomorrow," Mattingly said. "The guys I try to take care of, like Juan Uribe, Juan Rivera and Mark Ellis, I just can't throw out there every day."